Screening and Optimization of Fermentation Medium for Bacillus velezensis BP-1 and Its Biocontrol Effects against Peyronellaea arachidicola

In the submerged fermentation process, introduction of novel nutrient media as sources carbon and nitrogen can enhance antifungal activity. this study, we used a process to find optimal for Bacillus velezensis BP-1 strain grow in, that would boost its activity against Peyronellaea arachidicola. By using single-factor test central composite design (CCD) in response surface methodology, medium B. was identified. The antagonistic potential assayed P. arachidicola fungus manage web blotch disease peanut plant leaves. LB screened best medium, with maximum 90% comparison other mediums. Semolina flour source, root extract magnesium sulfate inorganic salt were selected components others. methodology optimized by 15 g/L semolina 13.68 0.50 salt, achieving inhibition vitro bioassays showed broth had significant activity, an rate 88.34% pot experiments on control, management effects revealed pre-inoculation spray efficiency (96%) when compared post-inoculation broth. These findings suggest strong This could be potent biocontrol tool aiding early plants.